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Signalway Antibody
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Merck KGaA
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Image Search Results
Journal: Frontiers in Neuroscience
Article Title: Additive Effects of Environmental Enrichment and Ketamine on Neuropathic Pain Relief by Reducing Glutamatergic Activation in Spinal Cord Injury in Rats
doi: 10.3389/fnins.2021.635187
Figure Lengend Snippet: Primary antibodies for western blotting.
Article Snippet:
Techniques: Western Blot
Journal: International Journal of Molecular Sciences
Article Title: EGCG, GCG, TFDG, or TSA Inhibiting Melanin Synthesis by Downregulating MC1R Expression
doi: 10.3390/ijms241311017
Figure Lengend Snippet: Effect of EGCG, GCG, TFDG, or TSA on expression of TYR, tyrosinase-related protein 1 (TRP1), and tyrosinase-related protein 2 (TRP2) mRNA and protein. B16F10 cells were pretreated with EGCG, GCG, TFDG, or TSA for 2 h and stimulated with α-MSH for 24 h in the presence of EGCG, GCG, TFDG, or TSA. The treatment group without α-MSH and a sample was referred to as CK1, and the treatment group with α-MSH but without a sample was referred to as CK2. ( a ) Cell lysates were prepared with an EASY spin Plus cell RNA rapid extraction kit. Total RNAs were subjected to qPCR analysis of TYR , TRP1, or TRP2 with β-actin as an internal control. Data are mean ± SD. ( b ) Protein extracts were prepared by lysing cells with radioimmunoprecipitation assay (RIPA) lysate containing protease inhibitors and phosphatase inhibitors. Total proteins were subjected to Western blot analysis of TYR, TRP1, or TRP2 with GAPDH as an internal control. Data are mean ± SEM; a,b,c,d,e,f,g different letters above the column indicate significant differences ( p < 0.05).
Article Snippet: Primary antibodies against TYR (TU343218),
Techniques: Expressing, Radio Immunoprecipitation, Western Blot
Journal: Cell Transplantation
Article Title: Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia
doi: 10.1177/09636897211041585
Figure Lengend Snippet: DOR activation with DADLE regulated p-synapsin I by BDNF-p-ERK pathway after global ischemia. Western blot analysis of BDNF (A), p-ERK1/2 (B), p-synapsin I (C) expression in rat hippocampus on day 3(left) and day 7(right) after global ischemia. ( N = 3 per group) * P < 0.05. ** P < 0.01. *** P < 0.001.
Article Snippet: After blocking with 5% skim milk, the membranes were incubated with primary antibodies against BDNF (1:100, Santa Cruz Biotechnology), p-ERK1/2, PKCα, β-actin, GAPDH (1:1000, Cell Signaling Technology),
Techniques: Activation Assay, Western Blot, Expressing