Journal: Frontiers in Neuroscience

Article Title: Additive Effects of Environmental Enrichment and Ketamine on Neuropathic Pain Relief by Reducing Glutamatergic Activation in Spinal Cord Injury in Rats

doi: 10.3389/fnins.2021.635187

Figure Lengend Snippet: Primary antibodies for western blotting.

Article Snippet: Rabbit anti-phospho-NMDAR2B Tyr 1472 (p-NR2B) , 1:1,000 , Merck Millipore, Darmstadt, Germany.

Techniques: Western Blot

Journal: Thoracic Cancer

Article Title: Patchouli alcohol induces G 0 / G 1 cell cycle arrest and apoptosis in vincristine‐resistant non‐small cell lung cancer through ROS ‐mediated DNA damage

doi: 10.1111/1759-7714.14982

Figure Lengend Snippet: Effects of patchouli alcohol (PA) on protein expression of DNA damage response signaling pathways. (a and b) A549 and A549/V16 cells were treated 112 μM and 135 μM PA for 0–48 h, respectively; the cells were then harvested and prepared for western blot analysis for p‐H2A.X, p‐CHK1, p‐CHK2, p‐p53, p21, CDK2, cyclin E1, ERK, p‐ERK, AKT, and p‐AKT. (c–h) The blots were quantified using ImageJ software, and data are expressed as mean ± SD. β‐actin was the loading control. * p < 0.05, compared with control.

Article Snippet: The specific primary antibodies used in this study included those against p‐H2A.X (GeneTex, USA), p‐CHK1 and p‐p53, p‐CHK2 (Biorbyt Ltd.), p21, CDK2, cyclin E1, ERK, p‐ERK, AKT, p‐AKT, Bcl‐2, Bax, caspase‐9, caspase‐3, CD133, CD44, EpCAM, β‐actin (iReal Biotechnology), and P‐gp (Bioss Antibodies Inc.).

Techniques: Expressing, Western Blot, Software

Journal: Journal of Assisted Reproduction and Genetics

Article Title: GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium – potential biomarkers in the detection of endometriosis – a pilot study

doi: 10.1007/s10815-020-01905-4

Figure Lengend Snippet: Changes in mRNA expression levels. Relative mRNA expression levels of GRN, NOTCH3, FN1, and PINK1 to the house keeping gene RPL0 . Bar graphs showing gene expression levels relative to the control group. Data represents mean fold change ± SEM. Control (without EM, n = 10); ASRM I (minimal EM, n = 10); ASRM II (mild EM, n = 10); ASRM III (moderate EM, n = 10). * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: The following kits were used: Progranulin ELISA Kit (No. E-EL-H1578, Elabscience, USA), Human Notch Homolog 3 ELISA Kit (No. SEL147Hu, Cloud-Clone Corp., USA), Quantikine ELISA Human Fibronectin (No. DFBN10, R&D Systems, USA), Human PINK1 ELISA Kit (No. MBS9327222, MyBioSource, USA).

Techniques: Expressing, Gene Expression, Control

Journal: Journal of Assisted Reproduction and Genetics

Article Title: GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium – potential biomarkers in the detection of endometriosis – a pilot study

doi: 10.1007/s10815-020-01905-4

Figure Lengend Snippet: Immunoreactivity and localization of proteins in eutopic endometrial tissue samples. Immunohistochemistry for GRN, NOTCH3, FN1, and PINK1. Data presents the mean of the intensity–reactivity score (IRS) of each cell type stained with the assessed proteins compared by the stage of EM. Bar graphs showing the typical localization of the determined proteins in eutopic endometrial tissue. IRS, intensity–reactivity score (product of intensity of staining and percentage of stained cells); intensity of staining: 0, none; 1, mild; 2, moderate; 3, high; percentage of stained cells: 0, none; 1, < 10%; 2, 10–50%; 3, 50–80%; 4 > 80%. Data represents the mean ± SEM. Control (without EM, n = 5); ASRM I (minimal EM, n = 5); ASRM II (mild EM, n = 3); ASRM III (moderate EM, n = 5)

Article Snippet: The following kits were used: Progranulin ELISA Kit (No. E-EL-H1578, Elabscience, USA), Human Notch Homolog 3 ELISA Kit (No. SEL147Hu, Cloud-Clone Corp., USA), Quantikine ELISA Human Fibronectin (No. DFBN10, R&D Systems, USA), Human PINK1 ELISA Kit (No. MBS9327222, MyBioSource, USA).

Techniques: Immunohistochemistry, Staining, Control